Stabilization of β-Catenin by Genetic Defects in Melanoma Cell Lines
摘要：
Signal transduction by β-catenin involves its posttranslational stabilization and down-stream coupling to the Lef and Tcf transcription factors. Abnormally high amounts of β-catenin were detected in 7 of 26 human melanoma cell lines. Unusual messenger RNA splicing and missense mutations in the β-catenin gene (CTNNB1) that result in stabilization of the protein were identified in six of the lines, and the adenomatous polyposis coli tumor suppressor protein (APC) was altered or missing in two others. In the APC-deficient cells, ectopic expression of wild-type APC eliminated the excess β-catenin. Cells with stabilized β-catenin contained a constitutive β-catenin-Lef-1 complex. Thus, genetic defects that result in up-regulation of β-catenin may play a role in melanoma progression.
展开
关键词：
Cell Line Tumor Cells, Cultured Animals Humans Mice Melanoma Cytoskeletal Proteins Adenomatous Polyposis Coli Protein DNA-Binding Proteins Trans-Activators
DOI：
10.1126/science.275.5307.1790
被引量：
年份：
1997
Semantic Scholar
万方医学
dx.doi.org
JSTOR
Oxford Univ Press
查看更多
NCBI
Europe PMC
AACR
JBC
ResearchGate
ProQuest
EBSCO
EBSCO
SAGE
jem.rupress.org
onacademic.com
mp.bmjjournals.com
ar.iiarjournals.org
go.galegroup.com
highbeam.com
cghjournal.org
biolreprod.org
ganino.com
(全网免费下载)
readcube.com
cancerci.com
www.socolar.com
fasebj.org
jimmunol.org
molbiolcell.org
emboj.embopress.org
genesdev.cshlp.org
nejm.org
mendeley.com
jacionline.org
ukpmc.ac.uk
sciencemag.org
dev.biologists.org
ganino.com
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
