A combined computational-experimental approach predicts human microRNA targets
摘要：
A new paradigm of gene expression regulation has emerged recently with the discovery of microRNAs (miRNAs). Most, if not all, miRNAs are thought to control gene expression, mostly by base pairing with miRNA-recognition elements (MREs) found in their messenger RNA (mRNA) targets. Although a large number of human miRNAs have been reported, many of their mRNA targets remain unknown. Here we used a combined bioinformatics and experimental approach to identify important rules governing miRNA-MRE recognition that allow prediction of human miRNA targets. We describe a computational program, "DIANA-microT", that identifies mRNA targets for animal miRNAs and predicts mRNA targets, bearing single MREs, for human and mouse miRNAs.
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关键词：
Animals Humans Mice Hela Cells MicroRNAs Base Sequence Nucleic Acid Conformation Genes, Reporter Binding Sites Computer Simulation
DOI：
10.1101/gad.1184704
被引量：
年份：
2004
万方医学
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掌桥科研
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