Structure of beta-glucan oligomer from laminarin and its effect on human monocytes to inhibit the proliferation of U937 cells.
摘要：
We analyzed the human monocyte-stimulating ability of laminarin from Eisenia bicyclis, lichenan from Cetraria islandica, and their oligomers depolymerized with endo-1,3--glucanase from Arthrobacter sp. The respective -glucan oligomers with different degrees of polymerization (DP) were fractionated from hydrolytic products of laminarin and lichenan using gel-filtration chromatography. The monocyte-conditioned medium pre-cultured in the presence of a fraction of -glucan oligomer (DP8) from laminarin exhibited inhibitory activity against the proliferation of human myeloid leukemia U937 cells, while those pre-cultured with other -glucan oligomers and the original laminarin and lichenan showed little or no activity. NMR analysis indicated that the -glucan oligomer (DP8) has an average DP value of 13, and its ratio of -1,3- to -1,6-linkages in glucopyranose units was estimated to be 1.3:1. These results indicate that the -1,3-glucan oligomer with a higher content of -1,6-linkage stimulates monocytes to inhibit the proliferation of U937 cells.
展开
DOI：
10.1271/bbb.69.553
被引量：
年份：
2005
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
