Definition and system implementation of strategies for method development of chiral separations in normal- or reversed-phase liquid chromatography using polysaccharide-based stationary phases

阅读量：

作者：

N Matthijs，C Perrin，M Maftouh，DL Massart，YV Heyden

展开

摘要：

This paper proposes strategies in normal- and reversed-phase liquid chromatography (NP-HPLC or NPLC and RP-HPLC or RPLC), which were developed using three polysaccharide-based stationary phases. Those strategies are implemented in a knowledge-based system for the chiral separation of drug enantiomers. Each strategy includes a screening and an optimisation stage. The screening stage allows a fast evaluation of separation possibilities and enantioselectivity for many drugs in a short period of time, while the optimisation stage gives the opportunity to enhance, if needed, the initially obtained separation. Different examples demonstrate the effectiveness of the strategies for fast method development.

展开

关键词：

Enantiomer separation Chiral stationary phases, LC Knowledge-based system Method development Polysaccharides

DOI：

10.1016/j.chroma.2004.04.040

被引量：

107

年份：

2004