Chemokines induced by infection of mononuclear phagocytes with mycobacteria and present in lung alveoli during active pulmonary tuberculosis.

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作者：

MI Sadek，E Sada，Z Toossi，SK Schwander，EA Rich

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摘要：

The capacity of () to induce production of chemokines with known chemotactic activity for monocytes and lymphocytes, the cellular building blocks of , was investigated. These chemokines included regulated upon activation, normal T expressed and secreted (RANTES), (), and (). stimulated production of and by blood monocytes (MN) and alveolar macrophages (AM). of MN and AM stimulated release but not production of RANTES. AM produced or released significantly higher levels than MN of RANTES (by 2.1-fold), (by 6.9-fold), and (by 5. 5-fold) (P < 0.05 for each). This study also confirmed that -infected AM produce the chemokine interleukin (IL)-8. of AM resulted in increased steady-state expression of messenger RNA (mRNA) for and and minimal increased expression of RANTES mRNA. Both an avirulent (H37Ra) and a virulent (H37Rv) strain of and purified derivative of H37Rv but not latex beads induced production of chemokines. Supernatants of -infected demonstrated chemotactic activity for both monocytes and lymphocytes partially inhibitable by neutralizing against the chemokines studied. Bronchoalveolar lavage fluid from patients with active as compared with healthy control subjects contained increased levels of RANTES (by 8-fold), (by 2.7-fold), and (by 8.9-fold) (P < 0.05), but not , as compared with healthy control subjects. Thus, multiple chemokines may be involved in recruitment of for in .

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关键词：

endothelium

DOI：

10.1165/ajrcmb.19.3.2815

被引量：

420

年份：

1998