Enzymatic Breakage and Joining of Deoxyribonucleic Acid VI. FURTHER PURIFICATION AND PROPERTIES OF POLYNUCLEOTIDE LIGASE FROM ESCHERICHIA COLI INFECTED WITH BACTERIOPHAGE T4
摘要：
Polynucleotide ligase has been purified 1000-fold from extracts of infected with bacteriophage T4. The enzyme catalyzes () the repair of phosphodiester bond scissions introduced into the strands of bihelical DNA by pancreatic DNase and () the conversion of hydrogen-bonded circular duplexes or concatemers of λ bacteriophage DNA to covalently bonded circular molecules or concatemers, respectively. The DNA products of these reactions have been characterized by sedimentation and end group analysis. The ligase does not catalyze the end to end joining of T7 bacteriophage DNA.
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关键词：
Coliphages Escherichia coli Magnesium Tritium Phosphorus Isotopes Sulfhydryl Compounds Deoxyribonucleases Ligases Adenosine Triphosphate DNA, Viral
DOI：
10.1016/0003-2697(68)90101-2
被引量：
年份：
1968
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