Structure of an E6AP-UbcH7 Complex: Insights into Ubiquitination by the E2-E3 Enzyme Cascade
摘要：
The E6AP ubiquitin-protein ligase (E3) mediates the human papillomavirus-induced degradation of the p53 tumor suppressor in cervical cancer and is mutated in Angelman syndrome, a neurological disorder. The crystal structure of the catalytic hect domain of E6AP reveals a bilobal structure with a broad catalytic cleft at the junction of the two lobes. The cleft consists of conserved residues whose mutation interferes with ubiquitin-thioester bond formation and is the site of Angelman syndrome mutations. The crystal structure of the E6AP hect domain bound to the UbcH7 ubiquitin-conjugating enzyme (E2) reveals the determinants of E2-E3 specificity and provides insights into the transfer of ubiquitin from the E2 to the E3.
展开
关键词：
Humans Angelman Syndrome Cysteine Ligases Ubiquitin-Conjugating Enzymes Ubiquitin-Protein Ligases Ubiquitins Crystallography, X-Ray Binding Sites Amino Acid Sequence
DOI：
10.1126/science.286.5443.1321
被引量：
年份：
1999
BMJ
Wiley
掌桥科研
万方医学
dx.doi.org
查看更多
NCBI
Europe PMC
JSTOR
NCBI
JBC
dialnet.unirioja.es
ProQuest
ResearchGate
EBSCO
EBSCO
embor.embopress.org
pdb.org
sciencemag.org
osti.gov
onacademic.com
jimmunol.org
www.socolar.com
jvi.asm.org
jn.physiology.org
molpharm.aspetjournals.org
rcsb.org
molbiolcell.org
biochemj.org
pubfacts.com
scripts.iucr.org
mendeley.com
elife.elifesciences.org
highbeam.com
jcb.rupress.org
biochemsoctrans.org
utexas.influuent.utsystem.edu
jacionline.org
jcs.biologists.org
fasebj.org
go.galegroup.com
science.sciencemag.org
(全网免费下载)
science.sciencemag.org
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
来源期刊
引用走势
站内活动
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
