Profiling protein function with small molecule microarrays
摘要：
The regulation of protein function through posttranslational modification, local environment, and protein-protein interaction is critical to cellular function. The ability to analyze on a genome-wide scale protein functional activity rather than changes in protein abundance or structure would provide important new insights into complex biological processes. Herein, we report the application of a spatially addressable small molecule microarray to an activity-based profile of proteases in crude cell lysates. The potential of this small molecule-based profiling technology is demonstrated by the detection of caspase activation upon induction of apoptosis, characterization of the activated caspase, and inhibition of the caspase-executed apoptotic phenotype using the small molecule inhibitor identified in the microarray-based profile.
展开
关键词：
DOI：
10.1073/pnas.172286899
被引量：
年份：
2002
国家科技图书文献中心
(权威机构)
万方
万方医学
掌桥科研
Semantic Scholar
(全网免费下载)
查看更多
dx.doi.org
Europe PMC
(全网免费下载)
NCBI
Europe PMC
NCBI
(全网免费下载)
NCBI
JSTOR
Pubmed Central
(全网免费下载)
adsabs.harvard.edu
ResearchGate
(全网免费下载)
ResearchGate
EBSCO
springerprofessional.de
mysciencework.com
zo.utexas.edu
(全网免费下载)
archive-ouverte.unige.ch
学术范
(全网免费下载)
pnas.org
pnas.org
(全网免费下载)
mcponline.org
mendeley.com
学术范
growkudos.com
stke.sciencemag.org
core.ac.uk
jn.nutrition.org
bio.utexas.edu
(全网免费下载)
f1000.com
onAcademic
deepdyve.com
The National Academy of Sciences of the United States of America
(全网免费下载)
Semantic Scholar
(全网免费下载)
NCBI
(全网免费下载)
Europe PMC
(全网免费下载)
Pubmed Central
(全网免费下载)
ResearchGate
(全网免费下载)
pnas.org
(全网免费下载)
bio.utexas.edu
(全网免费下载)
zo.utexas.edu
(全网免费下载)
学术范
(全网免费下载)
The National Academy of Sciences of the United States of America
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
引用走势
站内活动
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
