Electron microscopy of polar insertions in the lac operon of Escherichia coli
摘要：
Several strongly polar mutations in the omega region of the z gene of the lac operon result from insertions consisting of only two specific sequences of DNA, one about 870 and the other 1170 nucleotide pairs long (based on single-strand measurements). No sequence homology was detected between the shorter (IS 1 ) and longer (IS 3 ) insertions. The IS 1 insertion was shown to possess a specific attachment site, but it can be inserted with either orientation at several sites in the z gene. Four insertion sites in the omega region of gene z were identified and the position of the lac 5 substitution and the SR2 deletion in the λ plac DNA were determined by heteroduplex mapping.
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DOI：
10.1007/BF00333859
被引量：
年份：
1972
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