Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.
摘要：
Small amounts (7-250 pmol) of myoglobin, beta-lactoglobulin, and other proteins and peptides can be spotted or electroblotted onto polyvinylidene difluoride (PVDF) membranes, stained with Coomassie Blue, and sequenced directly. The membranes are not chemically activated or pretreated with Polybrene before usage. The average repetitive yields and initial coupling of proteins spotted or blotted into PVDF membranes ranged between 84-98% and 30-108% respectively, and were comparable with the yields measured for proteins spotted onto Polybrene-coated glass fiber discs. The results suggest that PVDF membranes are superior supports for sequence analysis of picomole quantities of proteins purified by gel electrophoresis.
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关键词：
Animals Membranes Rosaniline Dyes Myoglobin Lactoglobulins Amino Acid Sequence Methods Chromatography, High Pressure Liquid Polyvinyls Electrochemistry
DOI：
10.0000/PMID3611052
被引量：
年份：
1987
Elsevier
Taylor & Francis
BMJ
万方医学
NCBI
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