Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe.

来自万方医学

喜欢 0

阅读量：

412

作者：

B Ou，M Hampsch-Woodill，RL Prior

展开

摘要：

An improved method of oxygen radical absorbance capacity (ORAC) assay has been developed and validated using fluorescein (3',6'-dihydroxyspiro[isobenzofuran-1[3H],9'[9H]-xanthen]-3-one) as the fluorescent probe. Our results demonstrate that fluorescein (FL) is superior to B-phycoerythrin. The oxidized FL products induced by peroxyl radical were identified by LC/MS, and the reaction mechanism was determined to follow a classic hydrogen atom transfer mechanism. In addition, methodological and mechanistic comparison of ORAC(FL) with other widely used methods was discussed. It is concluded that, unlike other popular methods, the improved ORAC(FL) assay provides a direct measure of hydrophilic chain-breaking antioxidant capacity against peroxyl radical.

展开

关键词：

Fruit Peroxides Fluorescein Reactive Oxygen Species Phycoerythrin Fluorescent Dyes Antioxidants Sensitivity and Specificity Reproducibility of Results Food Analysis