摘要：

Flexible immobilized enzyme" model is proposed,that is: the flexible carrier is prepared by linking a hydrophilic and flexible polymer chain to the suface of the support,then enzyme is connected on the carrier by covalent bond Flexible immobilization have some following characteristics: it can meliorate disadvantage of enzyme inactivity caused by direct immobilization or by arm spacer immobilization and improve free degree of immobilized enzyme; monodisperse micro-spheres as support can profit homogenization in the immobilization reaction and enzyme catalysis reaction Dialdehyde starch as a flexible chain is connected on the carboxylized polystyrene support,flexible carrier is obtained and then papain is immobilized on this flexible carrier The activity recovery of immobilized papain can reach 50% equal to twice activity recovery of arm spacer immobilized papain with glutaric aldehyde

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