Rapid and reliable protein extraction from yeast.
摘要：
The methods currently used for protein extraction from yeast are either laborious or insufficiently reliable. Here I report a method for protein extraction for electrophoretic analysis that is both easy and reliable. In this method, yeast cells are subjected to mild alkali treatment and then boiled in a standard electrophoresis loading buffer. The method was tested for different strains of Saccharomyces cerevisiae and for yeast Hansenula polymorpha DL-1. It yields virtually complete extraction independently of the strain, growth conditions and protein molecular weight and allows working with small amounts of yeast cells grown on agar plates. Copyright ? 2000 John Wiley & Sons, Ltd.
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DOI：
10.1002/1097-0061(20000630)16:9<857::AID-YEA561>3.0.CO;2-B
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年份：
2010
Wiley
万方医学
掌桥科研
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