Recombinant escherichia coli and overproduction method of polyhydroxybutyrate using the strain
申请(专利)号:
US20040998473
申请日期:
2004-11-29
公开/公告号:
US2005227340A1
公开/公告日期:
2005-10-13
申请(专利权)人:
IN GYU KIM;IL LAE JUNG;HYO KOOK PARK;KUG CHAN KIM;BYUNG HO CHOI
国省代号:
KR
被引量:
摘要:
The present invention relates to a recombinant having phbCAB originated from and an overproduction method of polyhydroxyalkanoate using the same, more precisely, a recombinant 'MG1655/pTZ18U-PHB' or 'JIL938/pTZ18U-PHB' having phbCAB originated from and an overproduction method of polyhydroxyalkanoate comprising the steps of preparing transformed having phbCAB originated from , inoculating and culturing the cells (growth phase), inducing the production of polyhydroxyalkanoate in the recomibnant (stationary phase and producing phase), and inducing the extracellular secretion of the polyhydroxyalkanoate from the recombinant . The method of the present invention facilitates not only overproduction of polyhydroxyalkanoate such as polyhydroxybutyrate by a simple batch culture but also industrial use of biodegradable polyhydroxyalkanoate replacing conventional non-biodegradable plastics by its simple and easy fermentation, separation and purification process.
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