摘要：

A (1→6)-branched (1→3)-β-d-glucan (T-5-N), isolated from a m sodium hydroxide extract of the fruit bodies of Dictyophora indusiata Fisch., markedly exhibited anti-inflammatory effects on both carrageenan-induced edema and scalded edematous hyperalgesia in rat's hindpaws. The activities of T-5-N (25 mg/kg i.p. × 2) were more potent than those of phenylbutazone (25–50 mg/kg i.p. × 2). The conformational behavior of T-5-N was studied. Its molecular weight in neutral solution was about three times that in 0.25m sodium hydroxide. This finding, in addition to the results of optical rotatory measurement and complex-formation with Congo Red, indicated that T-5-N has an ordered, triple-helical structure in neutral or slightly alkaline solution (< 0.15m NaOH), and has single chains in highly alkaline solution (0.25m NaOH). The conformational transition occurs at concentrations of sodium hydroxide in the range of 0.15–0.25m.

展开