Two human glutamate decarboxylases, 65-kDa GAD and 67-kDa GAD, are each encoded by a single gene

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作者：

DF Bu，Erlander, M. G，Hitz, B. C，Tillakaratne, N. J，Kaufman, D. L，Wagner-McPherson, C. B，Evans, G. A，Tobin, A. J

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摘要：

We report the isolation and sequencing of cDNAs encoding two human glutamate decarboxylases (GADs; L-glutamate 1-carboxy-lyase, EC 4.1.1.15), GAD65and GAD67. Human GAD65cDNA encodes a Mr65,000 polypeptide, with 585 amino acid residues, whereas human GAD67encodes a Mr67,000 polypeptide, with 594 amino acid residues. Both cDNAs direct the synthesis of enzymatically active GADs in bacterial expression systems. Each cDNA hybridizes to a single species of brain mRNA and to a specific set of restriction fragments in human genomic DNA. In situ hybridization of fluorescently labeled GAD probes to human chromosomes localizes the human GAD65gene to chromosome 10p11.23 and the human GAD67gene to chromosome 2q31. We conclude that GAD65and GAD67each derive from a single separate gene. The cDNAs we describe should allow the bacterial production of test antigens for the diagnosis and prediction of insulin-dependent diabetes mellitus.

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关键词：

Medical Sciences γ-Aminobutyric Acid Insulin-Dependent Diabetes Mellitus Chromosome 2q Chromosome 10p

DOI：

10.1073/pnas.89.6.2115

被引量：

1138

年份：

1992