摘要：

Direct separation of racemic atenolol using normal _ phase HPLC with amide derivative as chiral stationary phase has been investigated. The effect of different content of 1,2 _ dichloroethane and methanol in mobile phase on the chiral separation was discussed. The effect of organic regulators on resolution was also studied. The optimized mobile phase for racemic atenolol is V(n _ hexane)V(1,2 _ dichloroethane)V(methanol) =68266. The resolution results of atenolol, propranolol and celiprolol were compared and the mechanisms of chiral separation were discussed.

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