Induction of Human Fetal Globin Gene Expression by a Novel Erythroid Factor, NF-E4
摘要：
The stage selector protein (SSP) is a heteromeric complex involved in preferential expression of the human gamma-globin genes in fetal-erythroid cells. We have previously identified the ubiquitous transcription factor CP2 as a component of this complex. Using the protein dimerization domain of CP2 in a yeast two-hybrid screen, we have cloned a novel gene, NF-E4, encoding the tissue-restricted component of the SSP. NF-E4 and CP2 coimmunoprecipitate from extract derived from a fetal-erythroid cell line, and antiserum to NF-E4 ablates binding of the SSP to the gamma promoter. NF-E4 is expressed in fetal liver, cord blood, and bone marrow and in the K562 and HEL cell lines, which constitutively express the fetal globin genes. Enforced expression of NF-E4 in K562 cells and primary erythroid progenitors induces endogenous fetal globin gene expression, suggesting a possible strategy for therapeutic intervention in the hemoglobinopathies.
展开
关键词：
Humans Fetal Blood Hematopoietic Stem Cells K562 Cells Macromolecular Substances Transcription Factors DNA Codon, Initiator Globins DNA-Binding Proteins
DOI：
10.1128/MCB.20.20.7662-7672.2000
被引量：
年份：
2000
Taylor & Francis
国家科技图书文献中心
(权威机构)
万方
掌桥科研
万方医学
查看更多
Semantic Scholar
dx.doi.org
NCBI
NCBI
(全网免费下载)
Pubmed Central
(全网免费下载)
NCBI
Europe PMC
JBC
NRC Research Press
ResearchGate
(全网免费下载)
ResearchGate
EBSCO
Citeseer
(全网免费下载)
mysciencework.com
deepdyve.com
physiolgenomics.physiology.org
emboj.embopress.org
bloodjournal.org
onAcademic
mcb.asm.org
mcb.asm.org
(全网免费下载)
mendeley.com
biolreprod.org
jem.rupress.org
core.ac.uk
www.socolar.com
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
来源期刊
引用走势
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
