The interactions between highly de-N-acetylated chitosans and lysozyme from chicken egg white studied by 1H-NMR spectroscopy

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作者：

Are，Kristiansen，Kjell，M.，Vrum，Hans，Grasdalen

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摘要：

We have investigated the binding interactions between highly de-N-acetylated chitosans and lysozyme from chicken egg white by one-dimensional and two-dimensional 1H-NMR spectroscopy. A fully de-N-acetylated chitosan (fraction of N-acetylated units, FA1H chemical shifts of lysozyme. However, a chitosan with FA=0.04, where the N-acetylated units are predominantly surrounded by de-N-acetylated units (a monoacetylated sequence), induced significant shifts of several lysozyme resonances, demonstrating a specific interaction between lysozyme and de-N-acetylated units in the chitosan. The interaction between the two positively charged molecules increased with increasing ionic strength, as expected. The dissociation constant (Kd) between lysozyme and the monoacetylated sequence was strongly dependent on pH* (pH measured in D2O), with Kd&#x2004

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关键词：

chitosan lysozyme ligand binding N -acetylated units SUP> 1 </SUP>H-NMR spectroscopy

DOI：

10.1046/j.1432-1327.1998.2510335.x

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年份：

1998