Directed deletion of a yeast transfer RNA intervening sequence.
摘要：
Many eukaryotic genes contain intervening sequences, segments of DNA that interrupt the continuity of the gene. They are removed from RNA transcripts of the gene by a process known as splicing. The intervening sequence in a yeast tyrosine transfer RNA (tRNA$^{ ext{Tyr}}$) suppressor gene was deleted in order to test its role in the expression of the gene. The altered gene and its parent were introduced into yeast by transformation. Both genes exhibited suppressor function, showing that the intervening sequence is not absolutely essential for the expression of this gene.
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DOI：
10.1126/science.6997991
被引量：
年份：
1980
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