Formation of Cytoskeletal Elements During Mouse Embryogenesis : II. Epithelial Differentiation and Intermediate-Sized Filaments in Early Postimplantation Embryos

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作者：

BRIAN，W.，JACKSON，CHRISTINE，GRUND，STEFANIE，WINTER，WERNER，W.，FRANKE

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摘要：

Following our study on the expression of cytokeratin filaments in preimplantation mouse embryos [30], we have examined the organization of cytoskeletal elements in early postimplantation embryos up to day 8 of gestation, employing electron microscopy, immunofluorescence microscopy and two-dimensional gel electrophoresis of cytoskeletal proteins labelled by incorporation of 35S-methionine. The characteristic epithelia formed by the embryonic ectoderm and proximal (visceral) endoderm present well-developed junctional complexes and various differentiated membrane structures. Several apical differentiations of the proximal endodermal cells, such as brush border-like microvilli, the endocytotic labyrinthum, and the supranuclear vacuoles resemble the organization of epithelial cells of the ileum of neonatal mammals. Both embryonic epithelia show typical desmosomes and attached intermediate sized filaments of the cytokeratin type. Other types of intermediate-sized filaments, such as vimentin

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关键词：

Animals Mice, Inbred ICR Mice Desmosomes Proteins Protein Precursors Microscopy, Electron Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique Keratins