摘要：

trated to3.5 ml, and diluted to 10ml with solution F (50mM NaHepes (pH 7.5), 1 mM dithiothreitol, 1%octyl 8-D-glucopyranoside,and 50 mM NaC1). The poolwas then loaded on a 5-ml hydroxylapatite column that had been equilibrated with solution F. The column was washed with 10 ml of solution F, and the fly subunits were eluted with a step gradient to 300 mM KPi (pH 7.5) in solution F. Protein concentrations were determined by staining with Amido Black (34).Assay of PIPLC Activity-Phospholipase C activity was measured

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