Macrophages secrete a novel heparin-binding protein with inflammatory and neutrophil chemokinetic properties
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150
摘要：
We report the identification and purification of a new inflammatory monokine synthesized by the macrophage tumor cell line RAW 264.7 in response to endotoxin. This monokine, which we term "macrophage inflammatory protein" (MIP), is a doublet with an apparent molecular mass of approximately 8,000 daltons on SDS-PAGE but forms aggregates of greater than 2 x 10(6) daltons as assessed by gel filtration. Partial NH2-terminal amino acid sequence data reveal no significant homology with any previously described protein. Although the monokine is anionic under physiological conditions, it is one of two major macrophage- secreted proteins that bind to heparin at high salt concentrations. At 100 ng/ml or greater, MIP is chemokinetic for human polymorphonuclear cells and triggers hydrogen peroxide production. Subcutaneous injection of 10 ng or greater of MIP into footpads of C3H/HeJ mice elicits an inflammatory response, characterized by neutrophil infiltration. These findings suggest that MIP is an endogenous mediator that may play a role in the host responses that occur during endotoxemia and other inflammatory events.
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关键词：
Animals Mice, Inbred C3H Mice Macrophages Inflammation Heparin Chemotactic Factors Biological Products Monokines Amino Acid Sequence
DOI：
10.1084/jem.167.2.570
被引量：
年份：
1988
BMJ
Semantic Scholar
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掌桥科研
万方医学
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