摘要：

Immune cell suspensions (100mL, contain- ing more than 103 cells) were added to a 250 mL of 15mM HEPES, containing 1 mM EDTA, 2 mM DTT, 3 mM MgCl2 (pH 7,05) and 20 mM D- glucose tracer labeled with 14C-glucose (1 mCi ). Immune cells were incubated at 37oC for 60 min and precipitated with two parts ice-cold 20 % TCA (v/v). Cell-protein bonded 14C-D-glucose was separated by filtration through "Millipore" (Sweden) filters (0,65 mm diameter), washed twice with 10 ml 20% TCA and 15 ml ethanol. Filter discs were dissolved in DMSO and bond- ed radioactivity determined using SL-4221 Intertechnique liquid scintillation counter (Roche Bioelectronique Kontron, France). Nonspecific bonding typically amounted to 10-20% of total bonded 14C-D-glucose.

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