Phosphorylated and nonphosphorylated forms of avian sarcoma virus polypeptide p19
摘要：
The major avian sarcoma virus phosphoprotein, p19, was found in virions in a phosphorylated and nonphosphorylated form. Both forms of p19 were immunoprecipitated with antiserum from hamsters bearing ASV-induced tumors, supporting previous claims that p19 is a viral RNA-encoded polypeptide. In addition, both forms contained the same methionine-labeled tryptic peptides. Antiserum raised against p27 was used to specifically immunoprecipitate [ 32P]- or [ 32S]methionine-labeled polypeptides from extracts of cells. A phosphoprotein which was specifically precipitated from extracts of infected cells comigrated with a similarly precipitated [ 35S]methionine-labeled polypeptide with a molecular weight of approximately 76,000. Moreover, the 76,000 MW phosphoprotein contains the same tryptic phosphopeptide as p19. The significance of these observations is discussed.
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DOI：
10.1016/0042-6822(77)90390-7
被引量：
年份：
1977
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