A method for purifying the platelet membrane glycoprotein IIb-IIIa complex
摘要：
A method has been developed for the rapid isolation of platelet membrane glycoproteins (GP) IIb and IIIa. This method produces an excellent yield and does not require the prior isolation of platelet membranes. Outdated platelets were washed and solubilized in Triton X-100. Concanavalin A affinity chromatography was used to purify a platelet glycoprotein fraction. The concanavalin A-retained glycoproteins were eluted and adsorbed with a heparin-Sepharose column to remove a major contaminant, thrombospondin. Sephacryl S-300 gel filtration was used as the final purification step to remove most fibrinogen and low-molecular-weight contaminants. Wheat germ agglutinin affinity chromatography was used to completely remove trace amounts of fibrinogen. The purified GP IIb and GP IIIa were analyzed by sucrose gradient sedimentation and found to consist of heterodimer complexes.
展开
关键词：
membrane glycoproteins glycoprotein purification platelet lectin affinity chromatography electrophoresis
DOI：
10.1016/0003-2697(85)90067-3
被引量：
年份：
1988
Elsevier
Wiley
万方医学
Semantic Scholar
(全网免费下载)
dx.doi.org
查看更多
Oxford Univ Press
Cell Press
NCBI
Europe PMC
adsabs.harvard.edu
ResearchGate
ResearchGate
(全网免费下载)
EBSCO
Citeseer
(全网免费下载)
mendeley.com
fertstert.org
atvb.ahajournals.org
mysciencework.com
deepdyve.com
bloodjournal.hematologylibrary.org
nejm.org
uncch.pure.elsevier.com
bloodjournal.org
dev.biologists.org
bloodjournal.org
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
