Introduction of new genetic material into pluripotent haematopoietic stem cells of the mouse
摘要：
An infectious retrovirus vector has been used to transfer a bacterial gene encoding resistance to the neomycin analogue G418 into pluripotent haematopoietic stem cells present in explanted murine bone marrow tissue. Subsequent transplantation of the cells into lethally irradiated mice results in engraftment of the animals with donor haematopoietic tissue containing the bacterial gene. This approach affords an efficient and rapid means of re-introducing genetically modified tissue into intact organisms and provides a system whereby the expression and regulation of cloned genes can be followed within the context of a well characterized developmental programme.
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关键词：
Animals Mice Hematopoietic Stem Cells Genetic Engineering Genetic Vectors Transformation, Genetic Bone Marrow Transplantation Radiation Chimera Retroviridae Genes, Bacterial
DOI：
10.1038/310476a0
被引量：
年份：
1984
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