A B cell-deficient mouse by targeted disruption of the membrane exon of the immunoglobulin |[mu]| chain gene

来自 Semantic Scholar

喜欢 0

阅读量：

408

作者：

D Kitamura，J Roes，R Kühn，K Rajewsky

展开

摘要：

Of the various classes of antibodies that B lymphocytes can produce, class M (IgM) is the first to be expressed on the membrane of the developing cells. Pre-B cells, the precursors of B-lymphocytes, produce the heavy chain of IgM (mu chain), but not light chains. Recent data suggest that pre-B cells express mu chains on the membrane together with the 'surrogate' light chains lambda 5 and V pre B (refs 2-7). This complex could control pre-B-cell differentiation, in particular the rearrangement of the light-chain genes. We have now assessed the importance of the membrane form of the mu chain in B-cell development by generating mice lacking this chain. We disrupted one of the membrane exons of the gene encoding the mu-chain constant region by gene targeting in mouse embryonic stem cells. From these cells we derived mice heterozygous or homozygous for the mutation. B-cell development in the heterozygous mice seemed to be normal, but in homozygous animals B cells were absent, their development already being arrested at the stage of pre-B-cell maturation.

展开

关键词：

B-Lymphocytes Animals Mice Immunologic Deficiency Syndromes Membrane Glycoproteins Receptors, Antigen, B-Cell Antigens, Differentiation, B-Lymphocyte Blotting, Southern Flow Cytometry DNA Mutational Analysis