Activation of transcription by IFN-gamma: tyrosine phosphorylation of a 91-kD DNA binding protein

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作者：

K Shuai，C Schindler，V Prezioso，J Darnell

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摘要：

Interferon-γ (IFN-γ) induces the transcription of the gene encoding a guanylate binding protein by activating a latent cytoplasmic factor, GAF (gamma-activated factor). GAF is translocated to the nucleus and binds a DNA element, the gamma-activated site. Through cross-linking and the use of specific antibodies GAF was found to be a 91-kilodalton DNA binding protein that was previously identified as one of four proteins in interferon-stimulated gene factor-3 (ISGF-3), a transcription complex activated by IFN-α. The IFN-γ-dependent activation of the 91-kilodalton DNA binding protein required cytoplasmic phosphorylation of the protein on tyrosine. The 113-kilodalton ISGF-3 protein that is phosphorylated in response to IFN-α was not phosphorylated nor translocated to the nucleus in response to IFN-γ. Thus the two different ligands result in tyrosine phosphorylation of different combinations of latent cytoplasmic transcription factors that then act at different DNA binding sites.

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关键词：

Animals Cell Line Cell Nucleus GTP-Binding Proteins Tyrosine Phosphotyrosine Oligodeoxyribonucleotides DNA-Binding Proteins Trans-Activators Interferon-alpha