detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes
摘要：
MicroRNAs (miRNAs) are 20–23 nucleotide (nt) RNA molecules that regulate gene expression post-transcriptionally. A key step toward understanding the function of the hundreds of miRNAs identified in animals is to determine their expression during development. Here we performed a detailed analysis of conditions fordetection of miRNAs in the zebrafish embryo using locked nucleic acid (LNA)-modified DNA probes and report expression patterns for 15 miRNAs in the mouse embryo.
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关键词：
Animals Zebrafish Mice Oligonucleotides Oligonucleotides, Antisense MicroRNAs Oligonucleotide Probes Gene Expression Profiling In Situ Hybridization Embryo, Mammalian
DOI：
10.1038/nmeth843
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Taylor & Francis
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