Visual Detection of Single-Nucleotide Polymorphism with Hairpin Oligonucleotide-Functionalized Gold Nanoparticles

来自 Semantic Scholar

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阅读量：

130

作者：

Yuqing He, Kang Zeng, Anant S. Gurung, Meenu Baloda, Hui Xu, Xibao Zhang and Guodong Liu

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摘要：

We report a simple, fast, and sensitive approach for visual detection of single-nucleotide polymorphism (SNP) based on hairpin oligonucleotide-functionalized gold nanoparticle (HOAu-NP) and lateral flow strip biosensor (LFSB). The results presented here expand on prior work ( Mao, X. , Xu, H. , Zeng, Q. , Zeng, L. , and Liu, G. Chem. Commun. 2009, 30653067.) by providing new approach to prepare HOAu-NP conjugates with a deoxyadenosine triphosphate (dATP) blocker, which shortens the preparation time of the conjugates from 50 to 8 h and lowers the detection limit 500 times. A hairpin oligonucleotide modified with a thiol at the 5′-end and a biotin at the 3′-end was conjugated with Au-NP through a self-assembling process. Following a blocking step with dATP, the hairpin structure of HO and dATP embed the biotin groups, and make the biotin groups in close proximity to the Au-NP surface, leading to the biotins being "inactive". The strategy of detecting SNP depends on the unique molecular recognition proper...

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关键词：

Epigenetics Histone demethylase (HDM JMJD2/KDM4 Fe(II) and 2-oxoglutarate (2OG) oxygenases AlphaScreen

DOI：

10.1021/ac101275s

被引量：

122

年份：

2010