How is the level of free arachidonic acid controlled in mammalian cells?
摘要：
Using a small substrate (thymidine 5'-(p-nitrophenyl phosphate) 3'-phosphate), the kinetics of staphylococcal nuclease insolubilized on CNBr-activated Sepharoses 4B and 6B are affected by internal diffusional limitations. Since we demonstrate that we are working under conditions in which external mass-transfer resistances do not influence the reaction rate, we propose a simple theoretical model that considers only the case of mixed enzymic reaction-internal diffusion kinetics. In the Eadie-Hofstee plots we find very good agreement between theory and experiment. The model accounts very well for the results obtained by changing support texture, reaction conditions, and/or enzyme concentration in the insoluble derivatives, variables that modify the diffusional restrictions of the system.
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关键词：
Homogeneous immunoassay immunoassay, homogeneous enzyme immunoassay immunoassay, enzyme antigens antibodies ligand binding separation-free enzyme immunoassay substrate and prosthetic group labels
DOI：
10.1042/bj2040003
被引量：
年份：
1982
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