Regulation of Activating Transcription Factor-1 and the cAMP Response Element-binding Protein by Ca/Calmodulin-dependent Protein Kinases Type I, II, and IV

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摘要：

The ability of activating transcription factor-1 (ATF1) or the cAMP response element-binding protein (CREB) to enhance transcription can be stimulated by increases in intracellular Ca2+ concentrations. To identify protein kinases which may mediate the ability of Ca2+ to activate these transcription factors, we compared the ability of constitutively active forms of several Ca2+/calmodulin-dependent protein kinases (CaM kinases) to activate ATF1 or CREB. We find that constitutively active CaM kinase I and IV can activate both ATF1 and CREB. In addition, expression vectors for full-length CaM kinase I and IV were able to augment the ability of Ca2+ influx to activate ATF1 or CREB consistent with a role for these kinases in mediating transcriptional responses to Ca2+ signaling. In contrast, CaM kinase II was unable to activate either ATF1 or CREB. These findings provide a potential mechanism that may permit variation in the ability of ATF1 and CREB to respond to changes in intracellular Ca2+ concentrations depending on differences in the relative concentrations of specific CaM kinases.

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Animals Cell Line Tumor Cells, Cultured Isoenzymes Transcription Factors Phosphopeptides Cyclic AMP-Dependent Protein Kinases DNA-Binding Proteins Saccharomyces cerevisiae Proteins Recombinant Fusion Proteins