Insertion of N regions into heavy-chain genes is correlated with expression of terminal deoxytransferase in B cells

来自 EBSCO

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作者：

SV Desiderio，GD Yancopoulos，M Paskind，E Thomas，MA Boss，N Landau，FW Alt，D Baltimore

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摘要：

The variable regions of immunoglobulin heavy chains are encoded in the germ line by three discrete DNA segments: V(variable) elements, D (diversity) elements and J(joining) elements. During the differentiation of B lymphocytes, individual segments from each group are brought together by recombination to form the complete VDJvariable region. To understand these processes better, we have now isolated and sequenced molecular clones representing intermediates (DJfusions) and final products ( V-to-DJjoins) of heavy-chain gene rearrangement in two cell linesthat represent analogues of cells at early stages of B-lymphocyte differentiation. Heavy-chain gene assembly in one cell line but not in the other is accompanied by the appearance of short nucleotide insertions at the recombinational junctions. The generation of such insertions is positively correlated with the expression of terminal deoxynucleotidyl transferase in these lines.

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DOI：

10.1038/311752a0

被引量：

1563

年份：

1984