Plumbagin, a Vitamin K3 Analogue, abrogates Lipopolysaccharide-Induced Oxidative Stress, Inflammation and Endotoxic Shock via NF-κB Suppression

来自 EBSCO

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作者：

R Checker，RS Patwardhan，D Sharma，J Menon，M Thoh，SK Sandur，KB Sainis，TB Poduval

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摘要：

Plumbagin has been reported to modulate cellular redox status and suppress NF-κB . In the present study, we investigated the effect of plumbagin on lipopolysaccharide (LPS)-induced endotoxic shock, oxidative stress and inflammatory parameters in vitro and in vivo . Plumbagin inhibited LPS-induced nitric oxide, TNF-α , IL-6 and prostaglandin-E2 production in a concentration-dependent manner in RAW 264.7 cells without inducing any cell death. Plumbagin modulated cellular redox status in RAW cells. Plumbagin treatment significantly reduced MAPkinase and NF-κB activation in macrophages. Plumbagin prevented mice from endotoxic shock-associated mortality and decreased serum levels of pro-inflammatory markers. Plumbagin administration ameliorated LPS-induced oxidative stress in peritoneal macrophages and splenocytes. Plumbagin also attenuated endotoxic shock-associated changes in liver and lung histopathology and decreased the activation of ERK and NF-κB in liver. These findings demonstrate the efficacy of plumbagin in preventing LPS-induced endotoxemia and also provide mechanistic insights into the anti-inflammatory effects of plumbagin.

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关键词：

endotoxemia cytokines MAPKinases GSH reactive oxygen species

DOI：

10.1007/s10753-013-9768-y

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年份：

2014