Interaction between the C. elegans cell-death regulators CED-9 and CED-4

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作者：

MS Spector，S Desnoyers，DJ Hoeppner，MO Hengartner

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摘要：

Programmed cell death (apoptosis) is an evolutionarily conserved process used by multicellular organisms to eliminate cells that are not needed or are potentially detrimental to the organism. Members of the Bcl-2 family of mammalian proteins are intimately involved in the regulation of apoptosis, but, their precise mechanism of action remains unresolved. In Caenorhabditis elegans, the Bcl-2 homologue CED-9 prevents cell death by antagonizing the death-promoting activities of CED-3, a member of the Caspase family of death proteases, and of CED-4, a protein with no known mammalian homologue. Here we show that CED-9 interacts physically with CED-4. Mutations that reduce or eliminate CED-9 activity also disrupt its ability to bind CED-4, suggesting that this interaction is important for CED-9 function. Thus, CED-9 might control C. elegans cell death by binding to and regulating CED-4 activity. We propose that mammalian Bcl-2 family members might control apoptosis in a similar way through interaction and regulation of CED-4 homologues or analogues.

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关键词：

Animals Apoptosis physiology Binding Sites Caenorhabditis elegans metabolism physiology Caenorhabditis elegans Proteins Calcium-Binding Proteins genetics metabolism Helminth Proteins genetics metabolism Mutagenesis Site-Directed Protein Binding Proto-Oncogene Proteins genetics metabolism Recombinant Fusion Proteins genetics metabolism Research Support Non-U.S. Gov't Research Support U.S. Gov't P.H.S. Saccharomyces cerevisiae genetics Sequence Deletion