摘要：

New strategies for utilizing sol-gel chemistry to improve sensor compatibility with physiological environments and produce xerogel membranes for novel optical sensor applications are described. Incorporation of nitric oxide (NO)-donor functionalities into a material is an established means of creating more biocompatible blood and tissue-contacting surfaces. A NO-releasing optical pH sensor designed for intravascular use was developed through covalent attachment of -diazeniumdiolate (NONOate) NO donors to aminoalkoxysilane-derived xerogels. A two-layer sensor configuration was employed wherein a NONOate-modified xerogel and porous tetramethyl orthosilicate xerogel containing a fluorescent pH indicator were sequentially dip-coated onto a tapered fiber-optic probe. The resulting sensors were capable of rapid and linear optical pH detection throughout the physiological pH range (7.0-7.8 pH) and generated NO fluxes >0.4 pmol cm sfor ∼16 h. Significant reductions of platelet adhesion to the NO-releasing materials versus controls were observed after exposure to platelet-enriched porcine plasma.

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