摘要：

Objective: To establish an HPLC method to directly separate clenbuterol enantiomers on α1-acid glycoprotein chiral column.Methods:The effects of chromatographic conditions including pH of mobile phase,ionic strength,type and concentration of organic modifiers,flow rate on chiral separation were studied.Results:The optimized chromatographic conditions were as follows:admixture of isopropanol-0.01 mol·L-1phosphate buffer(pH 5.0)(1:99)as the mobile phase,flow rate 0.8 mL·min-1,detection wavelength 213 nm,column temperature 25 ℃.Conclusions:The established method is proved to be fast and convenient.Clenbuterol enantiomers can be separated with high resolution factor by using α1-acid glycoprotein column.

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