Yeast tRNA3Leu gene transcribed and spliced in a HeLa cell extract.
摘要：
A cloned yeast tRNA3Leugene containing a 33-base intervening sequence (IVS) is selectively transcribed by a soluble extract from HeLa cells. The 130-nucleotide tRNA3Leuprecursor RNA formed is colinear with the gene and contains approximately 4 leader nucleotides and up to 9 trailer nucleotides. The IVS is accurately and efficiently removed by an endogenous HeLa excision-ligase activity to yield the spliced tRNA, the free IVS, and the half-tRNA intermediates. The splicing reaction occurs without prior 5′and 3′maturation of the precursor but, with this exception, this pattern of synthesis and subsequent maturation of the tRNA3Leuprecursor conforms to the scheme for tRNA biosynthesis deduced for the Xenopus system. Indeed, the two systems utilize similar or identical tRNA3Leuprecursors. Our results stress the extraordinary conservation of tRNA biosynthesis in eukaryotes and demonstrate that a HeLa extract provides a useful system for investigating this process.
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DOI：
10.1073/pnas.78.10.5963
被引量：
年份：
1981
Semantic Scholar
掌桥科研
万方医学
dx.doi.org
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