Caleosins: Ca2+-binding proteins associated with lipid bodies
摘要：
We have previously identified a rice gene encoding a 27kDa protein with a single Ca 2+ -binding EF-hand and a putative membrane anchor. We report here similar genes termed caleosins, CLO, in other plants and fungi; they comprise a multigene family of at least five members in Arabidopsis (At Clo 1–5). Northern hybridization demonstrated that At Clo 2–4 mRNAs levels were low in various tissues, while At Clo 1 mRNA levels were high in developing embryos and mature seeds. Analysis of transgenic Arabidopsis plants expressing the GUS reporter under control of the At Clo 1 promoter showed strong levels of expression in developing embryos and also in root tip cells. Antibodies raised against AtCLO1 were used to detect caleosin in cellular fractions of Arabidopsis and rapeseed. This indicated that caleosins are a novel class of lipid body proteins, which may also be associated with an ER subdomain.
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关键词：
Arabidopsis thaliana calcium-binding protein caleosin EF-hand gene expression lipid bodies vesicles
DOI：
10.1023/A:1026564411918
被引量：
年份：
2000
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