Nature of the antigenic complex recognized by T lymphocytes. I. Analysis with an in vitro primary response to soluble protein antigens.

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摘要：

In order to analyze the genetic factors involved in the regulation of macrophage-T-cells interaction we have developed an in vitro primary response to soluble protein antigens in which nonimmune T cells can be sensitized and subsequently challenged in tissue culture with antigen-pulsed macrophages. Antigen-specific , as measured by increased DNA synthesis, occurred when syngeneic antigen-pulsed macrophages were used for both initial and secondary challenge. No occurred when allogeneic antigen-pulsed macrophages were used for secondary challenge of cells primed when syngeneic macrophages. When allogeneic antigen-pulsed macrophages were used in both primary and secondary cultures it was difficult to assess antigen-specific stimulation due to the substantial mixed leukocyte reaction. However, when T cells from F1 were primed with parental antigen-pulsed macrophages they responded only to the parental macrophages used for initial but not to those of the other parent. These results are discussed with respect to T-of a complex antigenic determinant which may include I-region gene products.

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关键词：

Animals Guinea Pigs T-Lymphocytes Macrophages Antigens Epitopes Isoantigens Lymphocyte Activation Kinetics Solubility