Raptor, a binding partner of target of rapamycin (TOR), mediates TOR action.
摘要：
mTOR controls cell growth, in part by regulating p70 S6 kinase α (p70α) and eukaryotic initiation factor 4E binding protein 1 (4EBP1). Raptor is a 150 kDa mTOR binding protein that also binds 4EBP1 and p70α. The binding of raptor to mTOR is necessary for the mTOR-catalyzed phosphorylation of 4EBP1 in vitro, and it strongly enhances the mTOR kinase activity toward p70α. Rapamycin or amino acid withdrawal increases, whereas insulin strongly inhibits, the recovery of 4EBP1 and raptor on 7-methyl-GTP Sepharose. Partial inhibition of raptor expression by RNA interference (RNAi) reduces mTOR-catalyzed 4EBP1 phosphorylation in vitro. RNAi of C. elegans raptor yields an array of phenotypes that closely resemble those produced by inactivation of Ce-TOR. Thus, raptor is an essential scaffold for the mTOR-catalyzed phosphorylation of 4EBP1 and mediates TOR action in vivo.
展开
关键词：
Adaptor Proteins, Signal Transducing Animals Base Sequence Caenorhabditis elegans Carrier Proteins Cell Line Cloning, Molecular DNA, Complementary Enzyme Activation Gene Expression
DOI：
10.1016/S0092-8674(02)00833-4
被引量：
年份：
2002
Elsevier
Elsevier
(全网免费下载)
Taylor & Francis
Wiley
Semantic Scholar
查看更多
掌桥科研
万方医学
Semantic Scholar
(全网免费下载)
dx.doi.org
Ingenta
NCBI
Europe PMC
Oxford Univ Press
Cell Press
BioMed Central
NCBI
NRC Research Press
EureKaMag.com
(全网免费下载)
ResearchGate
EBSCO
mendeley.com
neoplasia.com
jacionline.org
jcs.biologists.org
msb.embopress.org
mct.aacrjournals.org
deepdyve.com
cancergeneticsjournal.org
onAcademic
biology.ucsd.edu
(全网免费下载)
jnutbio.com
core.ac.uk
(全网免费下载)
biochemj.org
mysciencework.com
molbiolcell.org
cghjournal.org
perspectivesinmedicine.cshlp.org
ajpcell.physiology.org
scienceopen.com
mcponline.org
hw-f5-msb.highwire.org
readcube.com
bloodjournal.org
infona.pl
classes.biology.ucsd.edu
(全网免费下载)
pubfacts.com
yadda.icm.edu.pl
f1000.com
www-biology.ucsd.edu
(全网免费下载)
Elsevier
(全网免费下载)
Semantic Scholar
(全网免费下载)
EureKaMag.com
(全网免费下载)
core.ac.uk
(全网免费下载)
classes.biology.ucsd.edu
(全网免费下载)
biology.ucsd.edu
(全网免费下载)
www-biology.ucsd.edu
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
