Isolation of caudal, a Drosophila homeo box-containing gene with maternal expression, whose transcripts form a concentration gradient at the pre-blastoderm stage

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摘要：

We report the isolation and characterization of caudal (cad), a previously unknown homeo box-containing gene from the 38E region on the left arm of the second . This homeo box has diverged from the prototype sequence in Antennapedia, but contains subregions which are highly homologous. By Northern analysis and in situ hybridization experiments two transcripts of 2.4 kb were found to accumulate in nurse and in the oocyte during . These transcripts generate a transient concentration gradient along the antero-posterior axis at the syncytial blastoderm stage. At the cellular blastoderm stage transcripts accumulate in a single band from 13-19% egg length at the posterior end. One zygotic transcript of 2.6 kb is detected. At later stages this transcript is localized in ectodermally and endodermally derived tissues such as the proctodeum, the Malpighian tubules and the posterior midgut. The 2.6-kb transcript is detectable until the onset of .

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关键词：

homeo box homeotic genes maternal expression RNA localization endoderm

DOI：

10.1002/j.1460-2075.1985.tb04030.x

被引量：

442

年份：

1985