Human kidney amiloride-binding protein: cDNA structure and functional expression.
摘要：
Phenamil, an analog of amiloride, is a potent blocker of the epithelial Na+channel. It has been used to purify the porcine kidney amiloride-binding protein. Synthetic oligonucleotides derived from partial sequences have been used to screen a human kidney cDNA library and to isolate the cDNA encoding the human amiloride-binding protein. The primary structure was deduced from the DNA sequence analysis. The protein is 713 residues long, with a 19-amino acid signal peptide. The mRNA was expressed in 293-S and NIH 3T3 cells, yielding a glycoprotein (i) that binds amiloride and amiloride analogs with affinities similar to the amiloride receptor associated with the apical Na+channel in pig kidney membranes and (ii) that is immunoprecipitated with monoclonal antibodies raised against pig kidney amiloride-binding protein.
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DOI：
10.1073/pnas.87.19.7347
被引量：
年份：
1990
万方医学
Semantic Scholar
掌桥科研
dx.doi.org
NCBI
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