摘要：

【Objective】To research the inhibiting effects of compound Chinese medicine against the avian influenza virus in vivo and in vitro, its pharmacokinetics and acute toxicity test, its effects on immune function of SPF chicken.【Method】 The effects of direct inactivation, preventive and therapeutic effects of compound Chinese medicine on the anti-avian influenza virus were studied on chicken embryo and MDCK cell. Its pharmacokinetics were studied by drug residual accumulation. The acute toxicity test of the compound Chinese medicine was studied by oral administration and intraperitoneal injection on Kunming mice. The effects ofthe compound Chinese medicine on immune function of SPF chicken was studied by means of the indexes of immune organs in chickens, the contents of CD3, CD4, CD8 and γδTCR by FAC and antibody titer of avian influenza virus detected in HI method. 【Result】The effective inactivate concentration, therapeutic concentration and preventive concentration of the compound Chinese medicine against the H5N1 and H9N2 avian influenza virus was 31.25 mg·ml-1, 125 mg·ml-1and 250 mg·ml-1, 31.25 mg·ml-1, 15.625 mg·ml-1 and 62.5 mg·ml-1 on chicken embryo, respectively. The effective inactivate concentration, preventive concentration of the compound Chinese medicine against the H5N1 avian influenza virus was 31.25 mg·ml-1, 125 mg·ml-1, but the therapeutic concentration was not detected, and 15.625 mg·ml-1, 31.25 mg·ml-1 and 31.25 mg·ml-1 against H9N2 on the MDCK, respectively. The concentration-time curves fitted the one compartment model and its equation was C=650.66e-4.9078t. The study of acute toxic experiments on mouse was as follows: After oral administration the compound medicine was safe. After intraperitoneal injection administration, the LD50 was 425.6mg·kg-1bw. The contents of CD3, CD4, CD8 and γδTCR was 64.01%, 27.39%, 45.05% and 32.19% after 21 days, respectively. The antibody titre of administration-immunity group was 1.6 titre higher than that of immunity group. The protective rate of preventive group was 30%, and 20% in the therapeutic group【.Conclusion】The compound medicine has a strong action of direct inactivation, preventive and therapeutic effects on the anti-avian influenza virus in vitro. Furthermore, it inhibited avian influenza virus adsorbed in cell surface and entered into intracellular. The pharmacokinetics parameter showed that its elimination was slow. It had a long time and it had long effect in the mouse. It enhanced cellular and humoral immune function and the indexes of immune organs in chickens. It could prolong the mean death time of chicken and took effect on the chicken challenged the subtype H5N1 of highly avian influenza virus in vivo. The compound Chinese medicine is preventive and safe, prolongs the mean death time of chicken and strengthens the immunity of chickens.

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