Molecular characterisation of the tyrosine tRNA genes of yeast

来自 Nature

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作者：

OLSON, MAYNARD V，MONTGOMERY, DONNA L，HOPPER, ANITA K，PAGE, GUY S，HORODYSKI, FRANK，HALL, BENJAMIN D

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摘要：

MANY of the tRNA genes of yeast are observable genetically as nonsense suppressors 1 . In particular, the set of unlinked suppressor loci SUP2 to SUP8 and SUP11 have been shown to insert tyrosine at nonsense codons in yeast iso-1-cytochrome c 2,3 . Direct biochemical evidence for the presence of suppressor tRNA in yeast strains bearing these suppressor genes has been obtained using in vitro translation systems 4,5 and by the demonstration that a new minor tyrosine tRNA species with a suppressor anti-codon appears in a SUP5 strain 6 . Although these findings suggest that there are eight different loci in yeast which code for tRNA tyr , only a single nucleotide sequence has been observed for tyrosine tRNA in wild-type yeast strains 7 . Thus it seems that all eight genes may share a common base sequence, at least in their structural regions. Because the yeast tRNA tyr SUP genes occupy known positions on the genetic map 8 and are complementary to a well characterised RNA molecule they are attractive candidates for molecular analysis. As a first step, we have labelled yeast tRNA tyr with 125 I and have hybridised this probe to electrophoretically fractionated Eco RI fragments of yeast DNA; eight Eco RI fragments of different sizes hybridise to tRNA t yr .

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plant breeding plant genetics

DOI：

10.1038/267639a0

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