摘要：

Freshly harvested allantoic fluid of PR8 (influenza) or Nagoya strain (HVJ) was centrifuged in a Spinco centrifuge. A condensed virus suspension with a titer of 20, 000 HA per ml was made from the deposit with saline. Sodium ethylmercurithiosalicylate (Merzonin, Takeda Co.) was added to the suspension in a final concentration of 1:10, 000. The virus particle was incubated at 37°C for 4 hours and kept in ice-box overnight and then washed twice in a Spinco with saline to remove Merzonin. Infectivity test of this Merzonin-virus with chick embryos indicated no viral propagation. Mice inoculated intranasally twice with a 0.1ml dose of Merzonin-virus in an interval of one hour died with extensive consolidation within four days. No infectivity of the consolidation lungs was confirmed with chick embryos. The lethal toxicity to mice of influenza virus or HVJ inoculated by intravenous or intracerebral route was lost by inactivation with Merzonin.We would propose, therefore, that a viral component which can cause the formation of consolidation is to be named "consolidation factor". The mechanism of production of pulmonary consolidation was discussed in connection with Reilly's phenomenon.Accelerated death of mice previously sensitized subcutaneously or intranasally with Merzoninvirus of influenza occured only when the Merzonin-virus was given intranasally, but dit not by other route. As the mice showed intensive bronchiospasm at autopsy, it seems to be that the acute death was the result of an allergic reaction following the attachment of virus particle to the sensitized respiratory epithelium.Relationship between the consolidation factor and the allergic antigen attributed to the particle was discussed.

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