RNA editing of the IQ domain in Ca(v)1.3 channels modulates their Ca-dependent inactivation.

来自 NCBI

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作者：

H Huang，BZ Tan，Y Shen，J Tao，F Jiang，YY Sung，CK Ng，M Raida，G Kã¶Hr，M Higuchi

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摘要：

Adenosine-to-inosine RNA editing is crucial for generating molecular diversity, and serves to regulate protein function through recoding of genomic information. Here, we discover editing within Ca(v)1.3 Ca channels, renown for low-voltage Ca-influx and neuronal pacemaking. Significantly, editing occurs within the channel's IQ domain, a calmodulin-binding site mediating inhibitory Ca-feedback (CDI) on channels. The editing turns out to require RNA adenosine deaminase ADAR2, whose variable activity could underlie a spatially diverse pattern of Ca(v)1.3 editing seen across the brain. Edited Ca(v)1.3 protein is detected both in brain tissue and within the surface membrane of primary neurons. Functionally, edited Ca(v)1.3 channels exhibit strong reduction of CDI; in particular, neurons within the suprachiasmatic nucleus show diminished CDI, with higher frequencies of repetitive action-potential and calcium-spike activity, in wild-type versus ADAR2 knockout mice. Our study reveals a mechanism for fine-tuning Ca(v)1.3 channel properties in CNS, which likely impacts a broad spectrum of neurobiological functions. Copyright 2012 Elsevier Inc. All rights reserved.

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关键词：

hydroperoxide lipids GUV photosensitization methylene blue

DOI：

10.1016/j.bpj.2011.11.1351

被引量：

134

年份：

2012