Genomic Instability in Mice Lacking Histone H2AX
摘要：
Higher order chromatin structure presents a barrier to the recognition and repair of DNA damage. Double-strand breaks (DSBs) induce histone H2AX phosphorylation, which is associated with the recruitment of repair factors to damaged DNA. To help clarify the physiological role of H2AX, we targeted H2AX in mice. Although H2AX is not essential for irradiation-induced cell-cycle checkpoints, H2AX-/- mice were radiation sensitive, growth retarded, and immune deficient, and mutant males were infertile. These pleiotropic phenotypes were associated with chromosomal instability, repair defects, and impaired recruitment of Nbs1, 53bp1, and Brca1, but not Rad51, to irradiation-induced foci. Thus, H2AX is critical for facilitating the assembly of specific DNA-repair complexes on damaged DNA.
展开
关键词：
Animals Mice, Knockout Mice B-Lymphocytes T-Lymphocytes Spermatocytes Cells, Cultured Chromosome Aberrations Histones Gene Targeting
DOI：
10.1126/science.1069398
被引量：
年份：
2002
万方医学
BioOne
Oxford Univ Press
NCBI
NCBI
查看更多
Cell Press
BioMed Central
Europe PMC
JSTOR
AACR
JBC
NRC Research Press
adsabs.harvard.edu
ResearchGate
(全网免费下载)
ProQuest
ResearchGate
Citeseer
(全网免费下载)
EBSCO
EBSCO
rstb.royalsocietypublishing.org
fasebj.org
plantcell.org
biochemj.org
highbeam.com
bloodjournal.org
biochemsoctrans.org
f1000.com
jcs.biologists.org
reproduction-online.org
readcube.com
sciencemag.org
biolreprod.org
www.socolar.com
onAcademic
science-open.com
ukpmc.ac.uk
erc.endocrinology-journals.org
pnas.org
emboj.embopress.org
cshperspectives.cshlp.org
ec.asm.org
mendeley.com
go.galegroup.com
jimmunol.org
jem.rupress.org
mcponline.org
molbiolcell.org
deepdyve.com
arthritis.pure.elsevier.com
cancergeneticsjournal.org
fertstert.org
d.scholar.cnki.net
elifesciences.org
scienceopen.com
pubfacts.com
ResearchGate
(全网免费下载)
Citeseer
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
