The T-cell-independent immune response to the hapten NP uses a large repertoire of heavy chain genes
摘要：
Hybridomas generated from C57BL/6 mice immunized with the hapten NP coupled to ficoll, a T-cell-independent carrier, produce monoclonal antibodies that use a large repertoire of VH regions and light chains. This contrasts with the homogeneity of the strain-specific response to NP observed with T-cell-dependent carriers, where most of the antibodies use a single VH region, V186.2, in combination with the lambda-1 light chain. There is no evidence for somatic mutation in any of the sequenced regions of the antibodies generated by NP-ficoll. Thus T cell participation is required for the homogeneity of the strain-specific hapten response, and probably for somatic mutation as well.
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关键词：
Animals Mice, Inbred C57BL Mice T-Lymphocytes Phenylacetates gamma-Globulins Ficoll Antibodies, Monoclonal Base Sequence Hybridomas
DOI：
10.1016/0092-8674(85)90244-2
被引量：
年份：
1985
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